Electrofusion and Culture of Protoplasts in Microfluidic Device and Delivery of Cells by Passive Pumping System

摘要：

The microfluidic device for electrofusion and culture of protoplasts was fabricated by using softlithography (for microchannel) and e-beam evaporation process (for electrode). The cells were transported by passive pumping method relying on surface tension. We have electrofused two cells which are Peucedanum japonicum protoplasts Glehnia littoralis protoplasts inside the PDMS based microfluidic channel.

关键词： Electrofusion Passive pumping Microfluidic device

作者: Jongil Ju Jung-Moon Ko Joong Yull Park Ju Yeoul Baek Hyeon-Cheol Cha SangHoon Lee

作者单位: Department of Biomedical Engineering, Korea University, Seoul, South Korea Korea Artificial Organ Ce Department of Biology, Dankook University, Cheonan, South Korea Department of Biomedical Engineering, Korea University, Seoul, South Korea Korea Artificial Organ Ce

会议类型: 国际会议

会议名称: The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)

会议地点: 沈阳

会议语种:英文

页码: 231-233

在线出版日期: 2007-10-21（万方平台首次上网日期，不代表论文的发表时间）