Genotyping by a Novel Alkaline Phosphatase-Mediated Allele-Specific Primer Extension on 1-D Microfluidic Beads Array for Prediction of Steroid Weaning

摘要：

This study reports the development of a novel alkaline phosphatase (originated from shrimp, AP)- mediated enzymatic approach for typing of single nucleotide polymorphisms (SNP) based on allele- specific primer extension and its application on fabricated one-dimensional (1-D) microfluidic beads array for prediction of steroid weaning. This novel on-chip beads extension approach could sensitize to 0.1pM quantities of synthesized DNA oligonucleotides at a SNR (signal-to-noise ratio)>2, that corresponded to an absolute detection sensitivity of~10<19> mol of target molecules in about 2μL sample. Also, this approach could simultaneously discriminate two polymorphisms C3435T and G2677T of MDR1 gene regarding the steroid weaning.

关键词： Microfluidics Beads array Alkaline phosphatase (shrimp) Steroid weaning

作者: He Zhang Xiao-Hai Yang Ke-Min Wang Wei-Hong Tan Jian-Hui Wen

作者单位: Biomedical Engineering Center, State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, China

会议类型: 国际会议

会议名称: The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)

会议地点: 沈阳

会议语种:英文

页码: 237-239

在线出版日期: 2007-10-21（万方平台首次上网日期，不代表论文的发表时间）