Microfluidic Cell Array with Concentration Gradient in Parallel Channels

摘要：

We present a microfluidic device, integrating a gradient generator by geometrically controlled diffusion and eight parallel lanes of microchannels with an array of sandbag microstructures built in each lane for the docking of suspension cells, that we have developed. Using the microfluidic device, an array of about 200 cells were exposed to differing concentrations of an analyte, with cellular responses captured within one snapshot of confocal image. It is also possible to multiplex the number of parallel microchannels with the gradient generator to increase the throughput of on-chip dose-dependent cell-based assays.

关键词： Microfluidic cell array Concentration generation Dose-dependent

作者: Tao Xu Cheuk-Wing Li Xin-Sheng Yao Guo-Ping Cai Meng-Su Yang

作者单位: Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China Key Laborat Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon Hong Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China

会议类型: 国际会议

会议名称: The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)

会议地点: 沈阳

会议语种:英文

页码: 250-251

在线出版日期: 2007-10-21（万方平台首次上网日期，不代表论文的发表时间）