Whole Blood PCR on a Static Chip Thermostat of ITO Substrate

摘要：

In this paper, PCR amplification on a static PCR chip system with whole blood sample without any purification was demonstrated. The static PCR chip system was developed based on a substrate of indium- tin oxide glass (ITO-glass) using the conductive layer as an electro-heating element. Pulse width modulation (PWM) and fuzzy proportion integration differentiation (PID) algorithms were adopted in controlling of temperature programs of the chip. Direct blood PCR amplification conditions, such as composition of reagents and thermal programs, were first studied systematically on GeneAmp PCR system, and on-chip PCR amplification from only 0.5μL blood was successfully demonstrated using the static PCR chip system. Only thermal treatment was involved in whole process. This work demonstrates that portable PCR chip system for point of care or in-field forensic analysis is possible.

关键词： PCR Whole blood Static PCR chip ITO glass

作者: Bai-Yan Qu Zhi-Yong Wu Kun Chen Fang Fang

作者单位: Research Center for Analytical Sciences, Northeastern University, Shenyang, China

会议类型: 国际会议

会议名称: The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)

会议地点: 沈阳

会议语种:英文

页码: 252-255

在线出版日期: 2007-10-21（万方平台首次上网日期，不代表论文的发表时间）