2-D Microfluidic Chemostat System for Long-Term Tracking of E. coli in Growing Population with Single-Cell Resolution

摘要：

To keep bacteria growing in a monolayer is important for studying the cellular dynamics of single cells in growing population. In this paper we report on a microfluidic system for long-term 2-Dimension culture of bacteria, which allows the bacteria to grow freely in a micro-channel without forming multilayer structure. The culture environment is well controlled to be constant and it can be easily changed from one to another if necessary. This system offers a versatile platform for many research fields such as gene expression dynamics, nois-induced diversity between individuals and familial phylogenetics.

关键词： 2-D culture Bacteria E. coli Chemostat Single-cell

作者: Lu-Ping Xu Wei-Ye Yao Yong Chen Francois Taddei Hang Ji Oi Ouyang

作者单位: The Center for Theoretical Biology of Peking University Physics School of Peking University Physics School of Peking University L Ecole Normale Superieure of Paris U571 of Inserm Paris

会议类型: 国际会议

会议名称: The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)

会议地点: 沈阳

会议语种:英文

页码: 259-261

在线出版日期: 2007-10-21（万方平台首次上网日期，不代表论文的发表时间）