An Integrated Microfluidic Device for Cell-Based High Content Screening
An integrated microfluidic device was described for cell-based high content screening (HCS), which enabled the multiparametric measurement of cellular responses in human liver carcinoma (HepG2) cells in response to several compounds in a high throughput format. This device consists of multiple drug gradient generators and parallel cell culture chambers, in which the processes of liquid dilution, micro-scale cell culture, cell stimulation and cell labeling can be integrated into a single device. This assay provides rich information involving plasma membrane permeability, nuclear size and mitochondrial transmembrane potential in anti-cancer drug-induced apoptosis of HepG2 cells, demonstrating the potential of this platform for application in the early stage of drug discovery.
Microfluidics High content screening Apoptosis Cellular assay
Nan-Nan Ye Jian-Hua Qin Wei-Wei Shi Xin Liu Bing-Cheng Lin
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
国际会议
The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)
沈阳
英文
262-263
2007-10-21（万方平台首次上网日期，不代表论文的发表时间）