Heat Shock Exposure Influences Tissue Variations in 70 kDa Heat Stress Proteins Expression of Common Carp
While the importance of heat shock proteins (HSPs) in the heat resistance of organisms is becoming widely accepted, the expression of HSPs in thermal tolerance may not be the same among different tissues. In this study, the common carp Cyprinus carpio was utilized to determine tissue-specificity of HSP70 proteins expression in vivo 24 h acute heat shock exposure. Upper thermal tolerance was firstly determined by a standard method to obtain the temperatures of acute heat shock. In exposure experiments, fish were kept at control for 10 ℃, or subjected to an acute heat shock for 24 h at 20 ℃, 24 ℃ and 28 ℃, or subjected to 20 ℃ and 28 ℃ heat shock and then allowed to recover, at control temperature for 24 h. Levels of HSP70 in the brain, kidney, gill, heart and muscle were determined by Western blots of one dimensional SDS-PAGE. Unlike heart, kidney, brain and gill appearing as a single band, the selected antibody recognized two protein bands in muscle. Prominent induction of HSP70 was detected in heart, gill and kidney following a 10–18 ℃ increase in temperature, while obvious decreased levels of HSP70 in gill was observed at 28 ℃ heat shock. These results suggest heat stress caused variations in the synthesis of HSP70 in different tissues of C. Carpio.
70-kDa heat shock protein biomarker fish heat stress stress protein temperature
WANG Yunbiao HOU Xiaoli XU Jingbo SHENG Lianxi ZHENG Yongchen
Department of Environmental Science and Engineering, Northeast Normal University, Changchun 130024, Architecture and Decoration College of Jilin Architecture and Civil Engineering Institute, Changchun Department of Central Laboratory, Second Hospital, Jilin University, Changchun 130041, Jilin, China
国际会议
2007环境科学与技术国际会议(The 2007 International Symposium on Environmental Science and Technology)
北京
英文
2007-11-13（万方平台首次上网日期，不代表论文的发表时间）