Accelerated Biodegradation of Diesel Oil by Hydrocarbon Degrading Bacteria Consortium Using Yeast Extract as Nitrogen Source
The aim of this study was to investigate the possibility of using yeast extract as an organic nitrogen source to enhance degradation of diesel oil, comparing with inorganic nitrogen source (NH4)2SO4. The 30-day biodegradation of diesel oil in aqueous solution experiments were carried out by a bacteria consortium using yeast extract and (NH4)2SO4 as nitrogen source, respectively. Except the different nitrogen sources, the biodegradation condition was same: 2％ inoculum quantity, 1％ diesel oil and 150 r/min shaking culture at 26℃. The residual n-alkanes in the aqueous solution were determined by gas chromatographic-mass spectrometric following the biodegradation. The biodegradation of main n-alkanes showed that yeast extract accelerated the biodegradation, almost 10 times faster than that of using (NH4)2SO4, though the ultimate biodegradation efficiency was same. The shortened lag phase indicates that yeast extract stimulates the growth of bacteria in the initial phase to increase the biomass, without reducing the ability of the consortium to degrade n-alkanes.
petroleum hydrocarbon degrading bacteria consortium nitrogen source diesel oil
TIAN Shengyan WANG Juan LIU Xianbin
College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin 300 College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin 300
国际会议
2007环境科学与技术国际会议(The 2007 International Symposium on Environmental Science and Technology)
北京
英文
2007-11-13（万方平台首次上网日期，不代表论文的发表时间）