Environmental Contaminants in Animals and Testicular Structure
Distribution of cadmium, lead, nickel and cobalt as a risk factor of environment, in testis and its effects on the testicular structure in experimental animals were studied. All studied elements were intraperitoneally administered in a single dose. Cadmium (CdCl2) was administrated for mice in group A/Cd (0.25 mg.kg-1 body weight) and group B/Cd (0.5 mg.kg-1). Rats received lead (PbNO3) in dose 50 mg.kg-1 (group A/Pb), 25 mg.kg-1 (group B/Pb) and 12.5 mg.kg-1 (group C/Pb) of body weight. Nickel (NiCl2) was administrated for mice in dose of 20 mg NiCl2 per kg of body weight (group A/Ni), 40 mg NiCl2 per kg b. w. (group B/Ni). Hamsters in group A/Co received cobalt (CoCl2) in dose 20 mg.kg-1 in group B/Co 10 mg.kg-1 and in group C/Co 5 mg.kg-1 CoCl2 /kg body weight. Animals were killed 48 hours after application of observed elements. After a preparation of histological samples the results were compared with control (Ctrl). In testis, significant dose-dependent accumulation of cadmium was found. Morphometric analysis found any significant differences in relative volume of germinal epithelium (89.08％-89.14％) and interstitium (10.86％-10.92％) of seminiferous tubules. The diameter of seminiferous showed insignificant dose-dependent decrease. Morphometric analysis showed that in all lead-treated groups the relative volume of seminiferous epithelium was significantly decreased. Relative volume of interstitium was significantly decreased in group C/Pb. The diameter of seminiferous epithelium was significantly decreased in group B/Pb and C/Pb in comparison with control. In both experimental groups with nickel a significant (p<0.001) decrease of germinal epithelium in comparison with control group was observed. Also a significant (p<.05) difference between groups A/Ni and B/Ni was detected. A relative volume of interstitium was higher but not significant by in both experimental groups. The increase in relative volume of lumen was registered in both experimental groups in comparison with control group without nickel. Morphometric analysis showed that in all cobalt-treated groups the relative volume of seminiferous epithelium was significantly decreased. In the relative volume of interstitium a significant increase was found between control group and experimental groups. After cobalt administration we have found linear non-significant decrease. The qualitative analysis of testicular tissues in all observed animals detected dilatation of blood vessels in interstitium, undulation of basal membrane and several empty spaces occurred in germinal epithelium. Our findings clearly suggest a negative effect of cadmium, lead, nickel and cobalt on the structure as well as on the function of seminferous epithelium in the place of spermatozoa production.
cadmium lead nickel cobalt testis toxic effects
LUKAC Norbert MASSANYI Peter KOVACIK Jaroslav TOMAN Robert KROCKOVA Jirina CIGANKOVA Viera KOESAROVA Adriana CAPCAROVA Marcela
University of Veterinary Medicine, Str. Komenskeho 73, SK-09001 Kosice, Slovak Republic Slovak Agricultural University in Nitra, Str A. Hlinku 2, SK-94976 Nitra, Slovak Republic
国际会议
2007环境科学与技术国际会议(The 2007 International Symposium on Environmental Science and Technology)
北京
英文
2007-11-13（万方平台首次上网日期，不代表论文的发表时间）