Determination and Analysis of Toluene Diisocyanate Metabolites in Mice Using Gas Chromatography-Mass Spectrometry
Objective To determine metabolites of toluene diisocyanate (TDI) in mice and deduced the pathway for toluene diisocyanate metabolism in the organism. Methods Toluene diisocyanate was administered to mice for 2 weeks, samples of blood, feces, and urine were taken, and the structure of the toluene diisocyanate metabolites was determined using gas chromatography-mass spectrometry (GC-MS). Conditions for TDI chromatography: Supelco PTETM-5 chromatographic column (30 mm × 0.25 mm × 0.25 μm); initial column temperature: 90℃, which was maintained for 30 min, after which the temperature was increased at a rate of 40℃/min to 280℃, which temperature was then maintained for 5.25 min; temperature for the vaporizing chamber: 250℃; carrier gas: helium flowing at 1.0 mL/min. Conditions for chromatography of TDI metabolites in the organism: 94％ methyl, 4％ ethenyl-bonded-phase fused-silica capillary column (30 + 2 mX 0.25 + 0.02 mm); initial column temperature: 30℃, which was maintained for 5 min, after which the temperature was increased at a rate of 80℃/min to 280℃, which temperature was then maintained for 5 min; temperature for the vaporizing chamber: 250℃; carrier gas: helium flowing at 1.0 mL/min. Conditions for mass spectrometry: EI for ionization; 70 eV for ionization energy; 280℃ for connecting tube temperature; 35-350 amu for range of scanning; and 1.0 μL for sample size. Results The results showed 2,4-toluene diisocyanate was metabolized into 2,4-diaminotoluene, which is completely the same in male and female animals. Conclusion Under the conditions selected for GC-MS, TDI metabolites in the organism can be isolated. Endogenous impurities in the blood, feces, or urine of mice do not interfere with the measurements.
gas chromatography-mass spectrometry (GC-MS) toluene diisocyanate metabolite
JI Yubin ZOU Xiang YU Lei LANG Lang LI Wenlan JI Chenfeng
Center of Research and Development on Life Sciences and Environmental Sciences, Harbin 150076, Heilongjiang, China
国际会议
2007环境科学与技术国际会议(The 2007 International Symposium on Environmental Science and Technology)
北京
英文
2007-11-13（万方平台首次上网日期，不代表论文的发表时间）