Enzymatic Degradation and Enhancement of Coarse Endoenzyme Activity Excreted from Flavobacterium SP FCN2
In this study, the enhancement of coarse endoenzyme activity excreted from Flavobacterium sp FCN2 for PAHs degradation was investigated. The results show:(1) Coarse endoenzyme excreted from Flavobacterium sp FCN2 could degrade anthracene, phenanthrene and pyrene effectively. Within 30 minutes, the removal rates of the three PAHs were 61.14％±3.03％, 30.63％±4.85％, and 40.53％±3.97％, respectively, indicating that the coarse endoenzyme had the highest removal efficiency for anthracene, and then pyrene and phenanthrene. (2) At 32℃ and pH 6, the endoenzyme was the most active. The coarse endoenzyme of Flavobacterium strains FCN2 had higher activity for degrading anthracene, phenanthtene, pyrene in weak acid medium at a temperature between 30℃ and 35℃. (3) Under the above mentioned optimal condition, various metal ions were investigated as additives which could potentially enhance enzyme activity. It was found that Ca2+ could markedly activate the coarse enzymes. As 0.5mmol/L Ca2+ was added, the removal rates of anthracene, phenanthrene and pyrene were improved by 1.34, 1.59 and 1.85 times, respectively. The Michaelis constants were 1.54×10-4 mol/L, 3.58×10-4 mol/Land 2.46×10-4 mol/L, respectively as 0.5mmol/L Ca2+ was added comparing with2.69×10-4mol/L, 4.28×10-4mol/L and 3.56×10-4 mol/L, respectively, without Ca2+ addition. PAHs enzymatic degradation accelerated by calcium addition can thus be proposed as an effective method for bioremediation of PAHs pollution.
Flavobacterium sp FCN2 PAHs endoenzyme metal ions
WU Manli NIE Maiqian WANG Xiaochang YANG Gangzhu SU Junmei
School of Environmental and Municipal Engineering, Xian University of Architecture and Technology, Xian 710055, Shanxi, China
国际会议
2007环境科学与技术国际会议(The 2007 International Symposium on Environmental Science and Technology)
北京
英文
2007-11-13（万方平台首次上网日期，不代表论文的发表时间）