Enzymatic Transformation: Anti-tumor Activated Products Derived from Waste of P. Notoginseng
Waste of P. Notoginseng to anti-tumor ginsenoside compound K (C-K) was studied. The enzymatic transformation products (ETPs) from the total saponins in leaves waste of Panax notoginseng (SLPN) was conducted by β-Glucanase, and an industrial enzyme β-glucanase was used to transform SLPN. The optimal experiment conditions of SLPN transformed were obtained as following: 20mg/mL substratum concentrations, 10％ enzymatic volume fraction, temperature 55℃, pH5.8, time 72h. ETPs were extracted by D101 macroreticular resin, separated and purified by column chromatographies on silica gel and thin layer gel. Two of the main compounds was identified as: ginsenoside C-K 20(S)-protopanaxadiol-20-O-β-D-glucopyranoside C-K and ginsenoside Mc 20(S)-protopanaxadio l-20-O-α- L-arabinofuranosyl(1→6)-β-D-glucopyranoside, the structure of C-K and ginsenoside Mc were elucidated on the basis of NMR spectral data. The results of inhibition growth on rats loaded with S180 sarcomata cell by ETPs and C-K showed that the inhibition tumor rate (ITR) of ETP and C-K were 41.8％ and 44.7％ by ETPs and C-K separately. The ant-itumor effects of ETP and C-K were very obviously.
waste Panax Notoginseng (Burk.) F.H.CHEN enzymatic transformation anti-tumor activity constituents β-Glucanase
JIANG Binhui WANG Li ZHAO Yuqing LIU Changfeng
College of Resources and Civil Engineering, Northeastern University, Shenyang 110004, Liaoning, Chin School of Environmental & Biological Engineering, Shenyang Institute of Chemical Technology College of Resources and Civil Engineering, Northeastern University, Shenyang 110004, Liaoning, Chin
国际会议
2007环境科学与技术国际会议(The 2007 International Symposium on Environmental Science and Technology)
北京
英文
2007-11-13（万方平台首次上网日期，不代表论文的发表时间）