Analysis of Profile of Bacterial Populations in a Soil Polluted with Anthracene
Contamination of soil with hydrocarbons occurs frequently in Mexico. Anthracene was used as a model to study the effect of polycyclic hydrocarbon aromatic (PAHs) on soil microorganisms and soil processes during a 76 days aerobic incubation in the laboratory. A DNA extraction technique was developed and amplification by PCR of the ribosomal fragment 16S followed by a separation on a acrylamide gel with desnaturalizante gradient (DGGE) was used to study changes in the soil microbial populations. The DNA obtained was used as template for the amplification of the gene 16S rDNA of 1.5 Kpb. A nested PCR was used to amplify a fragment of 0.5 Kpb with GC clamp and analyzed by DGGE. Genomic libraries of soil were used to identify the microbial species and to study the effect of hydrocarbon and the dissolvent on them.
agricultural soil antrhacene DGGE species identification
E. VAZQUEZ-NUNEZ L. DENDOOVEN R. MARSCH
Department of Biotechnology and Bioengineering, Cinvestav, México
国际会议
2007环境科学与技术国际会议(The 2007 International Symposium on Environmental Science and Technology)
北京
英文
2007-11-13（万方平台首次上网日期，不代表论文的发表时间）