Generation of Pesticides-degrading Genetically Engineered Bacteria Expressing the Protein of GFP and Carboxylesterase B1

摘要：

The genetically engineered bacteria, expressing the fusion protein of enhanced green fluorescent protein (EGFP) and carboxylesterase B1, have been successfully constructed, by cloning the genes of EGFP and carboxylesterase B1 into the pET-28b vector and then transforming E. coli BL21 (DE3). Expression of the fusion protein can be induced in E. coli BL21 (DE3) at 28℃ for 8h by even as little as 0.01mM isopropyl β-D-thiogalactopyranoside (IPTG). The genetically engineered bacteria not only can degrade pesticides in the environment but also can be found easily by fluorescence spectrophotometry. The green fluorescence emitted by EGFP in genetically engineered bacteria can be seen even by naked eye in daylight.

关键词： green fluorescent protein carboxylesterase B1 genetically engineered bacteria

作者: Li Qin Chen Rui Wu Yijun

作者单位: Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P. R. China

会议类型: 国际会议

会议名称: 第三届“农药与环境安全国际学术研讨会暨第七届“植物化学保护和全球法律一体化国际研讨会(3rd International Symposium on Pesticide and Environmental Safety & 7th International Workshop on Crop Protection Chemistry and Regulatory Harmonization)

会议地点: 北京

会议语种:英文

页码: 203-212

在线出版日期: 2007-10-09（万方平台首次上网日期，不代表论文的发表时间）