Construction of a Genetically Engineered Microorganism Possessing Biological Containment System and the Capability of Degrading Organophosphorus Pesticides
We constructed a genetically engineered microorganisms (GEMs) that can degrade organophosphorus pesticides (OPs), emit green fluorescence, and commit suicide on conditions. We designed two compatible plasmids and transformed them in one host. The green fluorescence character and the capability for degrading OPs were achieved by cloning the genes of EGFP and organophosphorus hydrolase (OPH) in the multiple cloning sites of plasmid pBV220. Thus, the fusion gene of EGFP and OPH, which was at the downstream of lambda P<,L> promoter, could be expressed freely as long as the GEM was metabolized. The efficient containment system was based on the conditional expression of a lethal gene that is extracellular nuclease gene of Serratia marcescens. The leadercoding sequence of the nuclease gene was deleted and the residual fragment was put under the control of the T7 promoter. The suicide cassette was placed in duplicate within a plasmid and responded to the presence of environmental signals arabinose. The containment system could be turned on in the presence of arabinose.
genetically engineered bacteria biological containment system organophosphorus pesticide degradation organophosphorus hydrolase
Li Qin Wu Yijun
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101 ,P. R. China
国际会议
北京
英文
213-224
2007-10-09（万方平台首次上网日期，不代表论文的发表时间）