Genome-wide gene expression changes in response to heavy metal exposure
Trace metals induce a variety of mechanisms in an organism that aim to protect cells from damage caused by free metal ions. One of the best studied single- gene protective mechanisms is due to the metal binding protein, metallothionein (MT). In recent years it has been shown that MT is only one of many genes induced by metal exposure. This paper reviews the available evidence coming from recent transcription profiling studies. Genome-wide scans using microarrays have demonstrated the involvement of a great variety of pathways, involving hundreds of genes, related to metal trafficking, antioxidant defence, sulphur salvage, signal transduction, protein folding, iron metabolism and the immune response. While most genomics studies are focused on model organisms with a fully sequenced genome, tools are also being developed for ecologically relevant non-models, such as the springtail Folsornia candida, used as an indicator organism for soil quality. For the aquatic environment Daphnia and fish are the most promising models. Genome-wide gene expression analysis will help us to make a big leap forward in understanding the complex interactions of trace metal ions with the living cell.
Collembola, Folsomia gene expression profiling genomics metallothionein soil stress response
Nico M. van Straalen Dick Roelofs Martijn J. T. N. Timmermans Janine Marien Thierry K. S. Janssens Muriel E. de Boer Ben Nora Tjalf E. de Boer
The Ecogenomics Team, Institute of Ecological Science, Vrije Universities, De Boelelaan 1085, 1081 H The Ecogenomics Team, Institute of Ecological Science, Vrije Universiteit, De Boelelaan 1085, 1081 H
国际会议
第九届痕量元素生物地球化学国际会议(9th International Conference on the Biogeochemistry of Trace Elements)
北京
英文
3-4
2007-07-15（万方平台首次上网日期，不代表论文的发表时间）