PIXE microanalysis of biological materials in frozen-hydrated state

摘要：

The first in-vacuum measurements of concentration and distribution of elements in biological specimens in the frozen-hydrated state using proton microbeam have been performed at Materials Research Group, iThemba LABS, South Africa. Micro-PIXE and proton backscattering were simultaneously used. Specimens showed good stability during analysis. Quantitative results were obtained by the standardless method tested with gelatin standards. An example of quantitative elemental mapping of frozen-hydrated specimens of the Ni-hyperaccumulating plant Senecio coronalus, compared with analysis of the same areas after freeze-drying indicated the same distribution patterns and comparable detection limits of the order of μg·g<-1> for most elements.

关键词： cold stage Ni-hyperaccumulation nuclear microprobe quantitative elemental distribution Senecio coronatus x-ray

作者: W. J. Przybylowicz G. Tylko J. Mesjasz-Przybylowicz A. Barnabas

作者单位: Materials Research Group, iThemba LABS, Somerset West 7129, South Africa Faculty of Physics and Appl Department of Cytology and Histology, Institute of Zoology, Jagiellonian University, 30-059 Krakow, Materials Research Group, iThemba LABS, Somerset West 7129, South Africa

会议类型: 国际会议

会议名称: 第九届痕量元素生物地球化学国际会议(9th International Conference on the Biogeochemistry of Trace Elements)

会议地点: 北京

会议语种:英文

页码: 338-339

在线出版日期: 2007-07-15（万方平台首次上网日期，不代表论文的发表时间）