Urinary 8-Hydroxy-2-deoxyguanosine as a biomarker for oxidative DNA damage in populations with long-term exposure to mercury
Mercury is a ubiquitous and highly toxic environmental pollutant. To study the relationship between mercury exposure and oxidative stress, serum and urinary Hg contents, oxidative DNA damage, and serum redox status have been evaluated in chronically Hg-exposed subiects and compared with the healthy controls. Urinary 8-hydroxy-2-deoxyguanosine (8- OHdG) content, which was used as a biomarker of oxidative DNA damage among the Hg-exposed people, was determined by HPLC coupled with electrochemical detection (ECD). The antioxidant status was evaluated by measuring the activities of superoxide dismutase and glutathione peroxidase and the concentrations of total reduced glutathione and protein-bound thiols in serum. Our data demon strate that Hg exposure can induce oxidative DNA damage, while the antioxidative repair systems can be expected to minimize DNA lesions caused by mercury. In addition, the results suggest that the determination of the urinary 8-OHdG content is a use ful way for evaluating in vivo oxidativeDNA damage in the Hg-exposed populations.
biomarker DNA damage mercury exposure
Chunying CHEN Yu-Feng LI Liya Qu Mei Li Bai LI Jiangxue WANG Yuxi GAO Zhifang CHAI
Key Laboratory of Nuclear Analysis Techniques, Institute of High Energy Physics, Chinese Academy of Guizhou Research and Designing Institute of Environmental Sciences, Guiyang 550004, P. R. China Key Laboratory of Nuclear Analysis Techniques, Institute of HighEnergy Physics, Chinese Academy of S
国际会议
第九届痕量元素生物地球化学国际会议(9th International Conference on the Biogeochemistry of Trace Elements)
北京
英文
464-465
2007-07-15（万方平台首次上网日期，不代表论文的发表时间）