Removal of tungstate from aqueous solutions by new strain Bacillus sp. GT-83 isolated from Anzali lagoon
(0) Attempt was made to isolate bacterial strains capable of biologically removing tungstate (WO<,4><2>). 38 water samples were collected from various areas of Anzali lagoon, Iran. Initial screening of a total of 100 bacterial isolates at pH 7, resulted in the selection of one isolate with maximum adsorption capacity of 52.1mg tungstate·g<1> dry weight. It was tentatively identified as Bacillus sp. according to morphological and biochemical properties and named strain GT-83. The Bacillus sp. GT-83 removed 72.1mg tungstate·g<1> dry weight within 30min from the solution with 100mg (WO<,4><2>)·L<1> initial concentration at pH 2, and thereafter adsorption rate decreased remarkably. Tungstate concentration was measured spectrophotometrically using dithiol method. Higher adsorption capacity was observed in acidic pH ranging from 1-3. Using 1mM sodium azide and 10mM 2, 4-dinitrophenol (2, 4 DNP), it was shown that only 30％ and 35％ reduction occurs in adsorption respectively and steam sterilization of the bacterial cells resulted in 20％ decrease in tungstate uptake. Equilibrium uptakes of tungstate increased with increasing tungstate concentration up to 300mg·L<-1>.
Anzali lagoon Bacillus sp biosorption oxyanions tungstate
S. Ghorbanzadeh Mashkani P. Tajer Mohammad Ghazvini
Department of Nuclear Biotechnology, Nuclear Research Center, Atomic Energy Organization of Iran, Tehran-Iran
国际会议
第九届痕量元素生物地球化学国际会议(9th International Conference on the Biogeochemistry of Trace Elements)
北京
英文
510-511
2007-07-15（万方平台首次上网日期，不代表论文的发表时间）