Survey of microbial life in a hydrothermal spring rich in arsenic colnpounds
Champagne Pool, one of New Zealands largest terrestrial hot springs and rich in metalloids such as arsenic and antimony compounds, has been subject to extensive geological and geochemistry descriptions. Only few microbiological studies (Ellis et al., 2005;Jones et al., 2001;Phoenix et al., 2005) recently described microbial activity based on culture-independent methods. In the current investigation, a combined approach of culture and culture independent studies was applied to investigate microbial life in Champagne Pool. Culture-independent methods such as microscopy, DNA yield, and ATP content confirmed relatively low biomass in Champagne Pool compared to other terrestrial hot springs within New Zealand and low cell numbers of (5. 6±0. 5)×10<6> ceils per ml were determined. Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA (small-subunit ribosomal nucleic acid) gene clone libraries analyses indicated low microbial diversity and the presence of Sulfurihydrogenibium, Nevskia, Paracoccus, Sulfolobus and Thermofilum-like populations in Champagne Pool. Three novel species were isolated and phylogenetic analysis of 16S rRNA gene sequences revealed relationships with members of the genera Thermoanaerobacter, Sulfurihydrogenibium, and Thermococcus. One of the isolate represents a novel genus and was able to grow in the presence of relatively high concentrations of arsenic and antimony compounds.
Antimony arsenic geothermal thermophilic microorganisms
A. Hetzer H. W. Morgan I. R. McDonald C. J. Daughney
Thermophile Research Unit, University of Waikato, Te Whare Wananga o Waikato, Gate 1 Knighton Road, Department of Biological Sciences, University of Waikato, Te Whare Wananga o Waikato, Hamilton, New Institute of Geological and Nuclear Sciences, Lower Hurt, New Zealand
国际会议
第九届痕量元素生物地球化学国际会议(9th International Conference on the Biogeochemistry of Trace Elements)
北京
英文
832-833
2007-07-15（万方平台首次上网日期，不代表论文的发表时间）