The Biological Effects of the Carbon Nanotubes and NanoTiO2 as well as Their Toxicities
The influences of the carbon nanotubes on proliferation state of chick embryo fibroblast (CEF) cells and toxicity of the nanotubes to the cells have been investigated by 3-(4,5- dimethylthiazol 2-yl)-2,5 diphenyltetrazolium bromide (MTT) colorimetric method and the toxicology. The carbon nanotubes are made by using a thermally chemical vapor deposition method in a gas mixture of nitrogen, hydrogen and acetylene. The diameters of the carbon nanotubes are measured by Scanning Electron Microscope and Transmission Electron Microscope, and are 100nm. These cells are divided into controlled and experimental groups. We found experimentally that the values of the cell proliferation rate of the CEF cells in the experimental group are very small, but the values of its relative cell proliferation rate are larger under the action of the carbon nanotubes when compared with that of the controlled group. This shows clearly that the influences of the carbon nanotubes on the proliferations of the CEF cells is very small, it is about first score. The surface properties and optical features of nanoTiO2 under actions of infrared and ultraviolet lights and its states in water solutions have been studied by Nicolet Nuxes FT-IF spectrometer and 970MC fluorescence spectrometer, etc., respectively. The nanoTiO2 is difficultly resolved in water, but its surface properties and biological activity are changed, when it interacts .with water molecules or ions and some organic small molecules with phosphoric acid functional groups in the solutions . A lot of new peaks in the infrared absorption and fluorescence spectra of the nanoTiO2 occur, when compared with bulk TiO2, for example, 1000nm powder. The changes of these properties make the nanoTiO2 have important applications in the industry, agriculture and medicine. The influences of nanoTiO2 on proliferation states of the persons liver cell has been observed and measured by MTT) method. The nanoTiO2 is prepared by chemical deposition method, its nanostructure is checked by Scanning Electron Microscope (SEM). We first measure some elementary properties of the nanoTiO2 and go on further the liver cell cultures, again add small nanoTiO2 into the cells. In accordance with biological rules we observe and measure the proliferation state of the liver cell in normal condition. The result obtained show that the influence of the nanoTiO2 on the proliferation of the persons liver cell is very small, when compared with that of the control group. We found further out the size of toxicity(safety) of the nanoTiO2 to the chick embryo fibroblast (CEF) cells by the MTT colorimetric method. Finally we determine the toxicity for the TiO2s of 100nm and of 1000nm by the toxicology which are all first score (or degree).
carbon nanotube MTT colorimetric method chick embryo fibroblast cell biological effect proliferation toxicity and persons liver cell
庞小峰 曾红娟 邓波
电子科技大学生命科学与技术学院纳米材料研究室,成都,610054
国际会议
成都
英文
2007-11-19（万方平台首次上网日期，不代表论文的发表时间）