Healing of periodontal defects treated with porous nanophase biphasic calciumphosphate ceramic -An experimental study in beagle dogs
Biphasic calciumphosphate (BCP) ceramic is an important bone repairing material in medical domain. The porous nanophase (material formulations with grain sizes less than 100 nm)BCP for the repair of periodontal defects, we studied, developed from an optimum balance of the stable phase of hydroxyapatite and the soluble β- tricalcium phosphate with the adjustable bioactivity and biodegradation. The objectives of this study are to investigate the effect of porous nanophase BCP ceramic implanted in periodontal defects as scaffold materials for tissue engineering, then to determine whether the material facilitate forming new bone tissue or not. 12 male Beagle dogs in the stage of permanent dentition were used in this study, 3 Beagle dogs acted as normal control, 9 dogs were removed buccal alveolar bone of bimaxillary second premolars (P2), all the defects equally were either implanted porous nanophase BCP ceramic or placed nothing to recover by themselves at random. Then divided the 9 dogs into 3 groups randomly,each group were 3，sacrificing one group at 4,8 and 12 weeks respectively，the same sacrificing period to 3 control dogs. Through non-destructive 3D micro-computer tomographic (μCT) imaging analysis to examine the morphology of porous scaffolds and the amount of newly formed bone 4,8 and 12 weeks after materials placement, then to analyze the bone regenerative procedures. After the histological observation, distinct changes like normal bone remodeling were detected in the areas filled with porous nanophase BCP ceramic, but not obvious in the self-recovery control group. It can be concluded the nanophase material could help to induce bone formation and promote bone healing in bone defects. The porous nanophase BCP ceramic is an excellent scaffold materials of bone tissue engineering, its function can be comparable with the natural bone, and extremely favorable for the growth of cells and bone tissue.
Nanophase Hydroxyapatite(HA) β-tricalcium phosphate (β-TCP) Bone regeneration Periodontal defect Micro-computer tomography
时函 赵宁 陈扬熙 廖运茂
四川大学华西口腔医院,成都,中国
国际会议
成都
英文
2007-11-19（万方平台首次上网日期，不代表论文的发表时间）