Magnetic Alginate Nanospheres with Doxorubicin as Targeting drug-Carriers Specificity for Hepatoma Carcinoma Cell
Magnetic alginate nanospheres (MANs) containing doxorubicin were prepared in an AOT/n-hexane reverse microemulsion system, then conjugated with Hab18, a monoclonal antibody by EDC, as targeting carriers for the antitumor drug. Results of structural analyses showed that the MANs containing 14.6％ of Fe, 10.8％ of doxorubicin and 65μg antibody per millgram of nanospheres were spherical morphologies with mean diameter of 185nm in a narrow distribution. The concentration of alginate, dosages of doxorubicin and Hab18 have the significant effects on the morphologies and diameters of MANs. The release time of the drug from the MANs lasted a long time. The experimental results of flow cytometer and ELISA showed that the immunological activity of the conjugated antibody on the MANs reached to 97.3％, and the antibody could target to SMMC-7721 tumor cells specifically. The MANs will act as the potential targeting carrier of anticancer drug in the near future.
Magnetic nanospheres alginate doxorubicin tumor targeting drug
李富荣 颜秋平 汤顺清 齐晖 任莉莉 周汉新
暨南大学第二临床医学院(深圳市人民医院)临床医学研究中心,深圳518020 暨南大学生物医学工程研究所;广州510632
国际会议
成都
英文
2007-11-19（万方平台首次上网日期，不代表论文的发表时间）