Morphological observation and adhesion properties measurement on human ovary carcinoma cells by AFM
Interaction between cells and extracellular matrix and measurement of mechanical properties of cells surface are discussed. By using AFM, two kinds of human ovary carcinoma cells (with highly invasive ability and lowly invasive ability) and its surrounding fibronectin fibrils are observed. The adhesion properties of both cells are investigated using AFM force-displacement technique. Results show: The shape of cells adhered to the glass slide is rectangular. At both ends of cells, there exit sucker-shape structures. Fibronectin fibrils around the cell are observed. Amount and distribution of surrounding fibrils of different kinds of cells are different. Number of fibrils around cells with highly invasive ability is less and their length is short. However, the surface has a higher adhesion force. Whereas, numbers of fibrils around cells with lowly invasive ability are more and their lengths are long. The adhesion forces at the middle part and the end part of cells with highly invasive ability are about 120nN and 210nN. The adhesion forces at the middle part and the end part of cells with lowly invasive ability are about 75nN and 105nN. On surface of both cells, the adhesion force at the end of the cell (similar to acetabulum) is greater than at the middle of the cell. These conclusions agree well with biological functions and properties of both cells. Moreover, experimental results indicate that the friction topography obtained by AFM can provide some useful information which can not be distinguished in the height topographies.
AFM human ovary carcinoma cell fibronectin fibrils adhesion force
韩毅敏 闫永达 孙涛 隋丽华 董申
哈尔滨医科大学附属第三医院妇科,哈尔滨,150040 哈尔滨工业大学精密工程研究所,413 信箱,哈尔滨,150001
国际会议
成都
英文
2007-11-19（万方平台首次上网日期，不代表论文的发表时间）