DNA detection with DNA chip based on Gold nanoparticles probes﹡

摘要：

DNA detection with DNA chip based on gold nanoparticles (AuNP) probes was introduced here. Gold nanoparticles were enriched 10 times by centrifugation. The amount of thiolated oligonucleotide used to modify gold nanoparticles could decrease to 1/10 than before enrichment. And this method couldnt affect the synthsis of AuNP probes as well as detection limit. The detection limit of DNA target with chip based on AuNP probes can reach to 10-12M. The hybridization signal is best with captured probes, detecting probes modified with gold nanoparticles and their complementary DNA target.

关键词： Gold nanoparticles probe DNA chip DNA detection

作者: 贾春平 金庆辉 毛红菊 赵建龙

作者单位: 中国科学院上海微系统与信息技术研究所 上海 200050

会议类型: 国际会议

会议名称: 第六届中国国际纳米科技研讨会

会议地点: 成都

会议语种:英文

在线出版日期: 2007-11-19（万方平台首次上网日期，不代表论文的发表时间）