The study on cell surface super-structure under the influence of virus infection
Apply AFM to observe the basic appearance of Han taan virus, make a imaging for Vero-E6 cells treated by glutaraldehyde and Vero-E6 cells infected by Han taan virus respectively, we observe the super-structure of Vero-E6 cells before and after infected by virus at the atomic or nanometer level.By Dropping the virus on mica sheet directly and scanning the virus after air drying), we can observe the structure of virus clearly; Cells were treated with glutaraldehyde of 0.5％～2.0％ concentration; The results of experiment revealed that the higher concentration of fixative we used, the better quality of pictures appeared, but the cells were damaged more seriously. The appearance of cells is similar to physiological conditions after reducing the concentration of fixative, and the quality of picture is also good. Cells were treated by glutaraldehyde of appropriate concentration after infected by different dilutions virus, then we observed that the appearance of cells varied greatly before and after infection, we also found that there are remarkable relationships between appearance changing of cells and concentration of virus.
AFM Han taan virus Vero-E6 cell
唐蕊华 薛小平 尹焕才 苏婧 谢玉为
西北工业大学生命科学院,西安 710072
国际会议
成都
英文
2007-11-19（万方平台首次上网日期，不代表论文的发表时间）