The changes of features of surface of ferroferric oxide nanoparticals and its absorption effects to Low density lipoprotein in blood
Iron oxide and its hydrate nanoparticles were synthesized by hydrothermal method and confirmed by infrared and SEM (Scanning Electron Microscope) et al. The dimensions of the nanoparticles are about 50-120 nm. The crystalline form of iron oxide nanoparticles is like globosity while its hydrate rod. Amino acids intermingling with the synthesized nanoparticles were crystallized to investigate the space effect of the nanoparticles. The crystalline forms of crystal are different to that of pure amino acid. The positions and width of the nanoparticles peaks in the infrared spectrum are changed too. Microscope observation and infrared spectrum results indicated the nanoparticles had changed the internal structure of amino acids crystal. To considerate the toxicity of the synthesized nanoparticles, MTT (3-(4,5-dimethylthiazol 2-yl)-2,5 diphenyltetrazolium bromide) assay was used. The OD value (Optical Density) was used to calculated RGR％ (Relative Generation Rate) of cells, which determined the grade of cytotoxicity. The RGR of nanoparticles of iron oxide and its hydrate are about 1 to 2, which indicate they have just low toxicity. Ferroferric oxide nanoparticles were synthesized by chemical coprecipitation method and confirmed by infrared and SEM et al. The dimensions of the nanoparticles are about 40-80 nm. The crystalline form of Ferroferric oxide nanoparticles is like globosity. MTT (3-(4,5-dimethylthiazol 2-yl)-2,5 diphenylte- trazolium bromide) assay was used to determine their cytotoxicity. The OD value (Optical Density) was used to calculated RGR％ (Relative Generation Rate) of cells, which determined the grade of cytotoxicity. The RGR of nanoparticles of ferroferric oxide is about 1, which indicate it has just low toxicity. Dextran Sulfate was graft on the surface of ferroferric oxide nanoparticals by NaOH at 60℃. The product was confirmed by infrared and SEM. The LDL (Low density lipoprotein) absorption experiment in vitro indicated its higher selectivity and adsorption with LDL. It may be a new method to reduce the level of LDL in the future.
Ferroferric oxide nanoparticles absorption effects Low density lipoprotein
庞小峰 邓波 赵强
电子科技大学生命科学与技术学院纳米材料研究室,四川,成都,610054
国际会议
成都
英文
2007-11-19（万方平台首次上网日期，不代表论文的发表时间）