Development Of Multiprotein Immunoassay System Using Micro Contact Printing And Microfluidic Techniques
We have developed simple soft lithographic techniques (microcontact printing and microfluidic devices) to create patterns of various proteins and immunoglobulins on various solid surfaces including glass, polystyrene & silicon with micron-size resolution. The various patterning techniques utilize the selective adsorption of proteins on surfaces with high hydrophobicity, direct microcontact printing of proteins using elastomeric stamps and microfluidic networks etc. These techniques have been found very useful and effective in creating two-dimensional patterns of proteins and immunoglobulins with microns size features. We have made square pattern arrays of goat IgGs, rabbit IgGs and human IgGs on hydrophilic glass/polystyrene substrate. The patterned IgGs acted as probes and could successfully immobilized corresponding anti IgGs. The fluorescent tagged anti IgGs could be examined with fluorescence microscopy.
soft lithography IgGs anti IgGs tagged with fluorescent dyes microcontact printing microfluidic network fluorescence microscopy immunoglobulins proteins
S. C. Jain Vikash Sharma
Liquid Crystals and SAM Section National Physical Laboratory Dr. K. S. Krishnan Marg New Delhi-110012
国际会议
北京
英文
2007-08-05（万方平台首次上网日期，不代表论文的发表时间）