Synthesis and characterization of lysine modified chitosan magnetic microspheres –a novel gene delivery system
This paper describes a new formulation of magnetic nanoparticles coated by a novel polymer lysine modified chitosan （CS-lys ）as gene carrier. Lysine modified chitosan was synthesized by performing carboxyl of lysine and amido of chitosan in the presence of 1-Ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride （EDC ）. The absolute chemistry of the Lysine modified chitosan obtained were characterized using IR and 1 H NMR, respectively. The results indicated that many amines of chitosan were modificatied with lysine,and optimized the correlation conditions. The lysine modified chitosan magnetic microspheres were preprated by co-precipitation method. The characterizations of microspheres were measured by TEM, XPS,Dynamic Laser Light Scattering, Vibrating Samples Magnetometer, and X-ray diffraction. The resultes indicated the average particle size was about 100nm and have good superparamagnetic property. The cytotoxicity of CS-lys MNPs was investigated with U293 cells. The results showed that the CS-lys MNPs retained of low toxicity; additionally, combination of DNA and CS-lys MNPs was observed from agarose gel electrophoresis, suggesting that the CS-lys MNPs could be a novel magnetic targeting gene carrier. We also studied the ability of complexes of CS-lys MNPs and DNA crossing BBB in rats by single photon emission computed tomography (SPECT).
Chitosan lysine magnetic microspheres target gene vector SPECT
Li Shuangyan Hao Lijuan Yang Yong Han Lei Yao Peng Huang Jie Chang Jin
Institute of Nanobiotechnology ,Tianjian University,Tianjin 300072,China
国际会议
海南三亚
英文
2007-01-10（万方平台首次上网日期，不代表论文的发表时间）