Role of IAP Gene of Bombyx mori in the Process of Baculovirus Infection

摘要：

Inhibition of apoptosis protein (iap) factor was amplificated from silkworm using RT-PCR. A recombinant baculovirus, carrying iap gene of Bombyx mori was successfully constructed for the large-scale production of IAP protein by Bac-to-Bac system. DNA of the recombinant baculovirus was transfected into Bm cells of the silkworm recombinant virus. Cell morphology was observed and cell survival rate was calculated at different time post infection. The results showed that the iap gene possessed anti- apoptotic role in the process of baculovirus infection. Over-expression of the iap gene at host cells could extend the life span and life cycle of virus.

作者: Wu Hui-ling LI Xi-bo Li Bing Shen Wei-de Wang Wen-bing

作者单位: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China School of Life Sciences, Soochow University, Suzhou 215123, China

会议类型: 国际会议

会议名称: 第六届中国国际丝绸会议暨第二届国际纺织高层论坛(The 6th China International Silk Conference and The 2nd International Textile Forum)

会议地点: 苏州

会议语种:英文

页码: 1-5

在线出版日期: 2007-09-13（万方平台首次上网日期，不代表论文的发表时间）