Expression Assay of Bombyx Mori Caspase-1 Gene in the Cells Treated with UV Irradiation and BmNPV Infection
In order to investigate the role that Caspase-1 plays in apoptosis signaling pathway of Bombyx mori (Bm), we established a real-time fluorescent quantitative PCR to detect the expression of caspase-1 in the Bm cells with different treatment. Ultraviolet (UV) irradiation (20mJ/cm2) and the infection of Bombyx mori nucleotide polyhedrosis virus (BmNPV) were applied to induce apoptosis in Bm cells, respectively. Bm cells were exposed in ultraviolet for 5s, and the expression of caspase-1 reached its peak at 10min after irradiation, which suggests that stimulation by ultraviolet increases the expression of caspase-1 gene in a short time. Inversely, viral infection reduced the expression of caspase-1 gene. Our results suggested that real-time fluorescent quantitative PCR used to detect the expression of caspase-1 is a reliable method and that Caspase-1 may be an upstream molecule in apoptosis pathway caused by UV irradiation and viral infection.
Wu Yan Wang Hai-xia Li Bing Shen Wei-de Wang Wen-bing
Institute of life sciences, Jiangsu University, Jiangsu Zhenjiang 212013, China School of Life Sciences, Soochow University, Suzhou 215123, China
国际会议
苏州
英文
6-9
2007-09-13（万方平台首次上网日期，不代表论文的发表时间）