Preparation of Silk Fibroin Film Cross-linked by Carbodiimide (EDC)
Regenerated silk fibroin film was prepared by using 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) as cross-linking agent and N- hydroxysuccinimide (NHS), 2-morpholinoethanesulfonic acid (MES) as assistant agent. The fine structure, water resistance, tensile strength and elongation at break and Youngs modulus of crosslinked film were examined and analyzed. The results showed that there were more silk I in crosslinked film when 10-15％ (relative to silk fibroin) EDC were added to silk fibroin solution and dried at 60℃. But the content of silk I decrease remarkably and the proportion of amorphous domains should correspondingly increase when the addition of EDC was more than 20％. The water solubility and Youngs modulus of crosslinked film decreased remarkably when 10-15％ (relative to silk fibroin) EDC were added to silk fibroin solution, while the elongation at break increased. NHS and MES could strengthen the effect of crosslinking as assistant agent.
silk fibroin film crosslinking
Zheng Zhong-hou Li Ming-zhong Zhao Ting Yan Shu-qin Cao Xu jia
College of Material Engineering, Soochow University, 178 East Ganjiang Road, Suzhou 215021, China
国际会议
苏州
英文
346-351
2007-09-13（万方平台首次上网日期，不代表论文的发表时间）