Capture and Release of Protein by Reversible DNA-induced Sol-gel Transition System
This paper introduces a novel sol-gel transition system that unitizes aptamer and DNA complementariy to achieve a controlled method of capturing and releasing-thrombin. In this system, a DNA-grafted polyacrylamide is gelatinized by a fuel DNA strand, which contains a thrombin-binding aptamer that binds according to base pair complementarity. This fuel DNA strand acts as a crosslinker strand that interweaves polymer chains to form a thrombin-seeded hydrogel. The advantage of having a DNA strand as a crosslinker is that another DNA strand, which is fully complementary to the crosslinker strand, can be applied to dissolve the hydrogel and release thrombin simultaneously. Moreover, the whole process is biologically compatible, since polyacrylamide is biocompatible. The potential applications of this study are in drug delivery, protein detection and protein purification.
Yongli Mi
Department of Chemical Engineering, Hong Kong University of Science and Technology, Hong Kong
国际会议
郑州
英文
7
2007-09-14（万方平台首次上网日期，不代表论文的发表时间）