Preparation and cell inclusion of fluorescent folic acid-functionalized mesoporous silica microspheres potentially useful for drug targeting
A hybrid fluorescent mesoporous silica material has been prepared starting from a mesoporous silica obtained by neutral surfactants. Synthesis was carried out according to a new interfacial procedure based on a not-catalyzed tetraethylorthosilicate hydrolysis aimed to tailor the particle size of the final product. As-synthesized materials have been derivatized with aminopropyl function partially coupled with fluoresceine isothiocyianate, a fluorescent tracer. Successively folic acid, a receptor-specific ligand has been covalently coupled to free aminopropyl groups. The resulting multifunctional material has been suspended in MCF-7 and HeLa cell cultures. Confocal microscopy showed cellular internalization in both cell lines, probably due to endocytosis.
L. Pasqua C. Morelli F. Testa D. Sisci E. Brunelli R. Aiello S. Andò J.B.Nagy
Dipartimento di Ingegneria Chimica e dei Materiali, Università della Calabria, Via Pietro Bucci, 870 Dipartimento Farmaco-Biologico, Università della Calabria, Via Pietro Bucci, 87030 Rende,Italy Dipartimento di Ecologia, Università della Calabria, Via Pietro Bucci, 87030 Rende, Italy Laboratoire de RMN, FUNDP, 61 rue de Bruxelles, 5000 Namur, Belgium
国际会议
北京
英文
2007-08-12（万方平台首次上网日期，不代表论文的发表时间）