Detection of Lactic Acid Bacteria against Streptococcus mutans and Purification of Its Bacteriocin
One strain designated KLDS 1.0432 of the isolated LABs was found to inhibit the growth of the indicator streptococcus mutans. Its supernatant fluid lost antimicrobial activity when treated by trypsin, pepsin and proteinase K, which indicated that the antimicrobial compound was protein or peptide. Analysis of the 16S rRNA gene sequence indicated that KLDS 1.0432 was a strain of Lactobacillus helveticus. The bacteriocin was purified by using the Hiload 26/60 Superdex 75 prep-grade chromatographic column and the AKTA purifier 100 system. Finally, Tricine- SDS-PAGE technology was used to estimate the molecular size of the bacteriocin and its size was found to be between 7823Da and 14400Da.
lactic acid bacteria streptococcus mutans bacteriocin
LIU Fang DU Lihui WANG Yutang HUO Guicheng
Key Lab. of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
国际会议
第五届科协博士年度科技会议(The 5th Annual Science Conference for Ph.D)
长春
英文
142-146
2007-08-29（万方平台首次上网日期，不代表论文的发表时间）