Development of one SCAR Marker for Detection of Sugarcane Smut Resistance
PCR amplification by random primers and bulked segregation analysis were occupied for screening of molecular marker linked to smut resistance or susceptible gone. A piece of random primer, which can amplify polymorphic fragments between smut resistant and susceptible varieties, was screened. The size of susceptible-specific fragment in susceptible varieties was about 600bp. The polymorphic fragment which appeared in susceptible bulked genomic DNA was cloned by pMD18-T vector. Positive recombinant clone identified by PCR amplification and restriction digestion was used for sequencing. The result of sequencing showed the fragment was 619bp long. Based on the sequence, a pair of primers with 23bp for upstream primer and 21bp for downstream primer was designed and a SCAR marker was developed (S619). In additional test with material of crossing parents, some crossing segregants and four standard varieties, which are used in classical identification by artificial inoculation, showed that results obtained by S619 was always the same as RAPD amplification. It indicated that this RAPD marker was converted into SCAR marker successfully and the SCAR marker is dominant. It would be potentially useful for identification of sugarcane smut resistance.
Sugarcane (Saccharum spp.) smut resistance RAPD SCAR
QUE Youxiong CHEN Tiansheng LIN Jianwei HAN Xinyun XU Liping ZHANG Muqing CHEN Rukai
Key Lab of Eco-physiology & Genetics Improvement for Sugarcane, Ministry of Agriculture, Fuzhou 350002, Fujian, China
国际会议
第五届科协博士年度科技会议(The 5th Annual Science Conference for Ph.D)
长春
英文
147-150
2007-08-29（万方平台首次上网日期，不代表论文的发表时间）