Phosphoinositide 3-Kinase and p38 MAPK Are Involved in bFGF-Indueed Endothelial Cell Migration
Objective: Migration of endothelial Cells is a key event in angiogenesis, Meanwhile, basic fibroblast growth factor (bFGF) is a potent angiogenic molecule. It has demonstrated that bFGF can induce migration of endothelial cells. However, the cell signaling pathways involved in bFGF-induced endothelial cells migration have not been shown yet. This study aimed to evaluate the impact of PI3Kinhibitor, wortmannin and p38 MAPK inhibitors, SB203580 and ERK1/2 inhibitor, PD98059 on bFGF-induced endothelial cells migration. Methods: Endothelial cells were isloated from the human umbilical vein by the explanted method. After bFGF (6ng/ml) treatment, the migration of endothelial cells was assessed by wound healing assay. Wortmannin (100ng/mol), SB203580 (10umollml), and PD98059 (20umol/ml) were used to inhibit the effects of bFGF respectively. Results: The distance of wound healing of bFGF-induced group was3.0+0.2, and that of Wortmannin group,SB203580 group and PD98059 group was6.6+0.2, 5.27+0.115, and 3.2+0.2 respectively. The results showed that bFGF (6ng/ml) resulted in a marked increase in the migration compared with the control (p<0.05), which was inhibited by Wortmannin (p<0.05) and SB203580 (p<0.05), but not PD98059 (p> 0.05). Conclusion: bFGF induced migration of endothelial cells. Phosphatidylinositol (PI) 3-kinase pathway andp38 MAPK pathway, but not ERK1/2 MAPK pathway, are likely involved in bFGF-mediated signal transduction leading to endothelial cell migration as well as carrying an anti-angiogenetic ability.
endothelial cells basic fibroblast growth factor migration Akt p38
Longju Chen Jinchang Lu Peijun Wang Jianghong Liang Hanqin Wang
Department of Anatomy, Yunyang Medical College, ShiYan 442000, HuBei, China Yunyang Medical College ,Affriliated Taihe Hospital ShiYan 442000, HuBei, China Department of Anatomy, Yunyang Medical College, ShiYan 442000, HuBei, China Institute of life scince
国际会议
首届国际断层影像解剖学研讨会(The 1st International Symposium on Sectional and Inaging Anatomy)
青岛
英文
209
2007-12-15（万方平台首次上网日期，不代表论文的发表时间）