An isolated fungal extra-cellular enzyme disintegrates the polysaccharide encapsulating a marine unicellular alga
A strain of Ref-1 was isolated from contaminant of micro-alga polysaccharide kept in a refrigerator and Strain of Ref-1 was identified as Homobasidiomycete. sp. It produced extra-cellular enzyme, the yield of which was enhanced more than 9.5 fold after selecting nutritional parameters. The maximum enzyme production was obtained at pH 3.5, 15℃, 5％ (w/v) haptose as carbon source and 0.5％ yeast extract as nitrogen source, and enzyme activity value obtained in liquid culture was 75 U/ml after 5 days of cultivation. The enzyme was optimally active at pH 5.0-5.5 and pH 7.5- 8.0, respectively, and stable over pH range 3.7-5.7 and 7.5-9.5. The enzyme optimally active at 55-75℃, remained at 91.9％ and 60.8％ of its original activity when incubated for 2 hours and 8 hours at 45℃, while it lost 55％ of its original activity after 30 min at 50-70℃, PH5.5. Ca<2+> of 10mM increased the activity by 2.0-folds, while Zn<2+> and Co<2+> of 10mM completely inhibited the enzyme activity. The specificity of substrates showed the enzyme from Ref- 1 had 94.1％ activity towards xylan, while no activity towards CMC, FP and starch.
polysaccharide Rfe-1 refrigerator Homobasidiomycete. sp
Z. L Xie Z. Y Cai Y. Mukohada
Qinghai university Xining, China 810001 Northwest Intitute of Plateau Biology, Chinese Ac ademy of Sciences Koch University of Technology, Japan
国际会议
第八届中韩菌物学研讨会(The Eighth China-Korea Joint Symposium for Mycology)
陕西杨凌
英文
121-139
2007-11-18（万方平台首次上网日期，不代表论文的发表时间）