An Optimization Framework for Overproducing Chemicals in Microbial Systems
On the past few decades, people are trying to develop renewable energy to reduce the rapid consumption of fossil resources. Therefore, metabolic engineering in microbial hosts for the production of renewable fuels and chemicals receives considerable attention. Because biotechnology changes with each passing day, many genome-scale metabolic models and data are available for organisms. In our preparation, we adopt some optimization models and stoichiometric matrix to formulate the problem of overproducing lactate. Stoichiometric matrix is useful for describing the flux balance. Optimization model determines the manipulation reactions. At the same time, some biological constraints have to add to the optimization problem, and we can get believable results which are close to real bio-systems. Consequently, we find the optimal solution of lactate yield accomplishing the maximum solution of biomass yield. Three strategies can get more than 90％ of the maximum theoretical lactate yield, and the flux distribution can be determined. We also find that the value of the regulation strength parameter would affect the production yield. When the value of regulation strength parameter increases, we can get a higher lactate yield. The proposed strategies could be provided on biochemical experiments, and then applied to industrial processes.
Metabolic engineering Optimization Metabolic pathway modeling
Ken-Ta Lee Cheng-Liang Chen
Department of Chemical Engineering,Taiwan University,Taipei
国际会议
西安
英文
2007-08-15（万方平台首次上网日期，不代表论文的发表时间）