Study on the immobilization of papain supported by HBS lignin

摘要：

The new idea of enzyme flexible immobilization was based on the bridge of a flexible and hydrophilic chain between the support and enzyme, so the rigidity and hydrophobic property of the support can be improved. If enzyme was immobilized on such a carrier with flexible chain, the immobilization can reduce the loss of enzyme activity and maintain the conformation of enzyme. In this paper, the papain was immobilized on the copolymer of HBS lignin grafted with flexible and hydrophilic polyacrylamide chain. The conditions of papain immobilization were investigated. The optimum conditions for immobilization were at 80℃, pH=8. 0 for 24h. The influences of polyacrylamide chain and the ratio of polyacrylamide grafted on the amount of immobilized papain were also discussed.

关键词： immobilized enzyme HBS lignin flexible immobilization papain acrylamide

作者: Jin Yanqiao Cheng Xiansu

作者单位: College of Material Science and Engineering, Fuzhou University, Fuzhou 350002, China College of Material Science and Engineering, Fuzhou University, Fuzhou 350002, China Department of C

会议类型: 国际会议

会议名称: 2006中国国际毛纺织会议暨IWTO羊毛论坛(2006 China International Wool Textile Conference & IWTO Wool Forum)

会议地点: 西安

会议语种:英文

页码: 402-404

在线出版日期: 2006-11-19（万方平台首次上网日期，不代表论文的发表时间）