Heavy Metal Ions Determination by Glucose Iosensor
The application of glucose biosensor to heavy metal ions detecting is introduced in this paper. An inhibition detection scheme has been employed for detecting Hg<2+> and Cu<2+> by an established glucose biosensor modified by Prussian blue. The Prussian blue modified electrode is used as the work electrode, the saturated calomel electrode is used as the reference electrode and another platinum electrode is used as the counter electrode. The circulation voltammetry method is used to scan the three electrodes systems. The influence factors such as pH of the buffer solution, detecting temperature and so on are researched to the glucose oxidase biosensor to detect Cu<2+> and Hg<2+>. The results show that, in a wider concentration scope, the peak value electric current and the concentration of the enzyme inhibitor assumes a good linear relationship respectively. When examines Cu<2+> and Hg<2+>, the linear response scope are 5~40μm and 2.5~22.5μm. The best response condition chooses the pH 6.86, 25℃. The above enzyme sensor may be used in these domains of toxic appraisal and environmental monitoring and so on.
Glucose oxidase biosensor heavy metal ions determination
Lan Lian Lin Wu Delian Yi Yuan Jiang Xiaorong Qin Yan Wang He Yang
Applied Chemistry Research Institute, Wuhan University of Science & Technology, China
国际会议
2006现代科技国际研讨会(The International Workshop on Modern Science and Technology in 2006)
北京
英文
151-154
2006-04-01（万方平台首次上网日期，不代表论文的发表时间）