Properties of Protein Adsorption on Carbonate-modified Hydroxyapatite
Carbonate-containing hydroxyapatites (HapS) were prepared at pH=7.0, 8.5, or 10.5 under CO<,2>-gas bubbling, or without it. Under CO<,2>-gas bubbling, calcium carbonate was predominantly produced at 8.5 and 10.5 of pH. On the other hand, only the XRD pattern of HAp was found in all the samples prepared without CO<,2> gas, and contained 1.74-4.40wt％ of carbonate. The amount of the carbonate increased as the increase in pH and IR spectra showed an OH- substituted carbonate species as well as PO<,4>- subsituted one with the increase of the amount. The adsorption properties of bovine serum albumin (BSA) were investigated at 295K on the samples of different contents of carbonate. All the adsorption isotherms followed the Langmuir-type adsorption indicating the monolayer adsorption. The saturated amounts of BSA decreased with the increase in the amount of carbonate in apatite, and this result was ascribed to the increase in the OH-substituted carbonate species, which located OH defect of the surface HAp, being an adsorption site of BSA.
Hydroxyapatite preparation of carbonate-containing hydorxyapatite apatite modification of the surface of apatite bovine serum albumin OH-substituted carbonate.
Tom Kanno Toru Sendai Kiyoshi Tada Jun-ichi Horiuchi Toshiyuki Akazawa
International Center, Kitami Institute of Technology, Japan Department of Applied and Environmental Chemistry, Japan Hokkaido Industrial Research Institute, Japan
国际会议
2006现代科技国际研讨会(The International Workshop on Modern Science and Technology in 2006)
北京
英文
228-233
2006-04-01（万方平台首次上网日期，不代表论文的发表时间）