Application of SSR Markers to Fingerprint Maize Hybrids and their Parental Lines
Four commercial hybrids in Indonesia (Semar-8, Semar- 9, Semar-10 and Bima-1), together with their parental lines, were analyzed using 26 SSR markers. The Semar varieties are. three-way cross hybrids and Bima-1 is a single cross hybrid. The objectives of this study were to ensure the genetic identity of the hybrids and their parental lines, and assess the purity of their inbred parents. Cluster analysis of data from 26 loci based on Jaccards similarity grouped the hybrids and their parental lines into two clusters. We identified 10 markers (phi109275, phi96100, phi374118, phi072, phi109188, phi299852, phi328175, phi233376, phi065 and umcl196) which were highly informative, and suitable for differentiating the hybrids and their parents. All the parental lines in this study could be distinguished using these markers. We identified three parental lines which have level of heterozygosity greater than: 20％. This study shows that SSR markers are reliable for assessing the genetic identity of maize inbred lines and hybrids.
Maize hybrids Parental lines SSR markers Fingerprinting
M. B. Pabendon M. J. Mejaya O. Suherman M. Dahlan Subandi
Indonesian Cereal Reseach Institute (ICERI), JI. Dr. Ratulangi Maros 90514, South Sulawesi, Indonesia
国际会议
第九届亚洲玉米大会(the Ninth Asian Regional Maize Workshop)
北京
英文
86-88
2005-09-05（万方平台首次上网日期，不代表论文的发表时间）