Early Response and Molecular Mechanism of Submergence Tolerance in Maize Roots Using Microarray and Suppression Subtractive Hybridization Analysis
In this study, two inbreds with a difference in submergence tolerance, Mo17 and Hz32, were used to reveal early responsive genes in root cells under submerged conditions. Suppression Subtractive Hybridization (SSH) and cDNA microarray analysis showed that a number of gene expressions were altered under the submergence treatment. These genes are involved in a broad spectrum of biochemical, cellular, and physiological processes, such as glycolysis, energy metabolism, lipid metabolism, signal conduction, DNA transcription, protein biosynthesis and digestion, cellular components and photosynthesis. The spectrum of genes in the first 0.5h of treatment was significantly different from that of the genes induced 2～4h later. In addition, gene expression profiling in response to submerging was different in two inbred lines. Four transcription factors were expressed in different profiles in Hz32 and Mo17. The metabolic and physiological conversions from aerobic to anaerobic conditions were faster in Hz32 than in Mo17. Based on the responsive genes and their functions, we propose that early responses of maize roots to submergence stress may be a complex network involving multiple physiological and metabolic pathways, and regulation of transcriptional processes plays a critical role in metabolic adaptation of maize roots during early stages of submergence.
Maize Zea mays Submergence stress Suppression subtractive hybridization SSH cDNA microarray Regulation of gene expression
Zuxin Zhang Xiling Zou Wanhu Tang Yonglian Zheng
College of Agronomy, Hebei Agricultural University, Baoding, Hebei 071001, P. R. China National Key National Key Laboratory of Crop Genetic and Improvement, Huazhong Agricultural University, Wuhan 430
国际会议
第九届亚洲玉米大会(the Ninth Asian Regional Maize Workshop)
北京
英文
140-143
2005-09-05（万方平台首次上网日期，不代表论文的发表时间）