The Characterization of Manganese Superoxide Dismutase mRNA from Ganoderma microsporum

摘要：

We have cloned and sequenced a gene (Mn-Sod) encoding manganese superoxide dismutase from Ganoderma microsporum. The deduced mature manganese-superoxide dismutase has 200 amino acids without putative mitochondrial-targeting signal peptide. The deduced amino-acid sequence includes conserved residues required for enzymatic activity and metal binding, and shares significant similarity with Mn-containing superoxide dismutase. Amino acid sequence analysis shows that the G. microsporum MnSOD is closely related to Mn-SODs from Paxillax involutus. And genomic DNA sequence analysis revealed a 744-bp partial ORF containing three introns with fungal consensus splice-site junctions.

关键词： Ganoderma microsporum manganese superoxide dismutase mRNA

作者: Ya-Hui Huang Ching-Tsan Huang Huei-Fang Wang Ruey-Shyang Hseu

作者单位: Department of Agricultural Chemistry, National Taiwan University, Taipei, China

会议类型: 国际会议

会议名称: 2002国际灵芝专题研讨会(International Symposium on Ganoderma Research)

会议地点: 北京

会议语种:英文

页码: 58-66

在线出版日期: 2002-10-21（万方平台首次上网日期，不代表论文的发表时间）