Purification and Identification of Glycopeptides from Ganoderma lucidum Fruit Bodies Cultivated with Grass and Wood Log
This research studied the amount and properties of glycopeptides of Ganoderma lucidum cultivated with wood log (GLPW) and grass (GLPG), and investigated the feasibility of substitution of grass for wood log in cultivate Ganoderma lucidum. Glycopeptides were extracted and isolated from fruit bodies of Ganoderma lucidum followed by dialysis. The component sugar and molecular weight distribution of the glycopeptides were determined by GC and HPLC. The structure of the glycopeptides was detected by IR, <1>H NMR and <13> C NMR. The molecular weight of the glycopeptides of GLPW and GLPG was Mw 5.13×10<5> and 5.85×10<5>,Mη 6.21× 10<5> and 6.96×10<5> respectively. The glycopeptides from both GLPW and GLPG were linked mainly through β-glycosidic bond and consisted of 16 amino acids as well as Rha, Xyl, Fru, Gal and Glc (There was small amount of Man in GLPW). The physical properties and chemical components of GLPW and GLPG were very similar (or have minimal differences). The glycopeptide extracting rate of GLPG was 2.8 times to GLPW.
Glycopeptide Ganoderma lucidum extraction identification
Shu-Qian Lin Sai-Zhen Wang Zhi-Bin Lin Yue-Xin Lin
Fuzhou Green Valley Biological pharmaceutical Technology Institute, Fuzhou, China Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing, China Fujian Teachers University, Fuzhou, China
国际会议
2002国际灵芝专题研讨会(International Symposium on Ganoderma Research)
北京
英文
109-114
2002-10-21（万方平台首次上网日期，不代表论文的发表时间）