Comparison of the Effects by Polysaccharides from Wood-cultured and Bag-cultured Ganoderma lucidum on Murine Spleen Lymphocyte Proliferation in vitro

摘要：

This research compared the influences of wood- cultured Ganoderma lucidum polysaccharides (GL-PS-WC) and bag-cultured Ganoderma lucidum polysaccharides (GL-PS-BC) on the proliferation activities of murine spleen lymphocytes in vitro, and investigated whether GL-PS-BC could be substituted for GL-PS-WC. Mixed lymphocyte culture (MLC) reaction, lymphocyte proliferation induced by concanavalin A (Con A, 1μg· mL<-1>) or lipopolysaccharide (LPS, 5μg·mL<-1>), MLC reactions inhibited by immunosuppressive drugs, Cyclosporine A (Cs A, 0.1μg·mL<-1>), Mitomycin C (Mit C, 0.1μg·mL<-1>), or antitumor drug, Etoposide (VP-16, 0.1μg·mL<-1>), were detected in the presence or absence of GL-PS-WC and GL-PS-BC at a concentration range of 0.2～12.8μg·mL<-1>. Two kinds of polysaccharides were shown to promote MLC at the range of 0.2～ 12.8μg·mL<-1>, increase lymphocyte proliferation induced by Con A or LPS and antagonize the inhibitory effects of CsA, Mit C or VP- 16 on MLC. No significant difference was observed between these two kinds of polysaccharides at selected concentrations. GL-PS- WC and GL-PS-BC showed similar effects on the proliferation activities of murine spleen lymphocytes in vitro.

关键词： Ganoderma lucidum polysaccharides (GL-PS) mixed lymphocyte culture (MLC) lymphocyte proliferation

作者: Li-Zhen Cao Zhi-Bin Lin

作者单位: Department of Pharmacology, Health Science Center, Peking University, Beijing, China

会议类型: 国际会议

会议名称: 2002国际灵芝专题研讨会(International Symposium on Ganoderma Research)

会议地点: 北京

会议语种:英文

页码: 115-121

在线出版日期: 2002-10-21（万方平台首次上网日期，不代表论文的发表时间）