Free Radical Scavenging Activity of Ganoderma lucidum Polysaccharides Peptide (GLPP) in the Mice Peritoneal Macrophages
The scavenging effects on free radicals of Ganoderma lucidum polysaccharides peptide (GLPP) were investigated. Alloxan and tert-butylhydroperoxide (t- BOOH) were used as an oxidant to injury mice peritoneal macrophages in vivo or in vitro respectively. 2, 7-dichlorodihydrofluorescein diacetate (DCHF-DA) was used as fluorescent probe. The fluorescence from cells was observed under the confocal microscope. Time series scan of confocal microscope was used to observe the changes of fluorescence by GLPP in mice peritoneal macrophages. The result showed that GLPP had free radical scavenging activity in the mice peritoneal macrophages.
Ganoderma lucidum polysaccharides peptide free radicals peritoneal macrophages DCHF-DA
Yu-Hong You Zhi-Bin Lin
Department of Pharmacology, School of Basic Medical Science, Peking University Health Science Cente Department of Pharmacology, School of Basic Medical Science, Peking University Health Science Center
国际会议
2002国际灵芝专题研讨会(International Symposium on Ganoderma Research)
北京
英文
141-145
2002-10-21（万方平台首次上网日期，不代表论文的发表时间）