Extraction and Purification of Polysialic Acid from fermentation broth
Sialic acids belong to a family of neuraminic acid (5-amido-3, 5-dideoxy-D- glycero-D -galacto -nonulosonic). N-acetylneuraminic acid (NeuAC). The representative member of this family is an important molecule in the biological recognition systems. Polysialic acid is a homopolymer of sialic acid. It is found only in a few animal glycoproteins and in certain bacterial polysaccharides. Currently, larger amounts of NeuAC-based novel pharmaceutical agents and diagnostic reagents for influenza viruses are in high demand for medical applications. In addition, polysialic acid has potential application in controlled-drug release as polysialic acid can prolong the active life and improve the pharmacokinetics of conventional drugs and biologicals. Polysialic acid was first recovered from the E. coli fermentation broth by adding ethanol. Addition of 4％ NaCl reduces the amount of ethanol usage. The precipitate was obtained by centrifugation followed by reconstitution in distilled water. The solution subsequently passed through a cartridge packed with Kieslguhr diatomite or pearlite to remove the cells and some proteins. The filtrate was adjusted to pH 10 and then passed through a cartridge packed with Kieslguhr diatomite or pearlite again to remove some more basic proteins and other impurity. The filtrate was collected and precipitated again with ethanol. The filtrate was dissolved and treated with CPC (cetyl pyridinium chloride). Polysialic acid solution was again treated with ethanol and freeze–dried using a lyophilizer. The purity of the polysialic acid product reached 90％ and protein content was below 1％。
Polysialic acid Sialic acid Extraction Purification
Xiaobei Zhan Jianrong Wu Danfeng Yu Jinlong Liu Zhiyong Zeng
School of Biotechnology, Jiangnan University, Wuxi 214122 China
国际会议
The 5th International Conference on Separation Science and Technology(第五届国际分离科学与技术会议)
北京
英文
2007-10-14（万方平台首次上网日期，不代表论文的发表时间）