Preparative isolation of Isorhamnetin from stigma maydis using a high-speed countercurrent chromatography
Preparative high-speed countercurrent chromatography (HSCCC) has been used successfully for the isolation and purification of Isorhamnetin from stigma maydis. This was achieved in two stages. The first stage used HSCCC protocol with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (HEMW) with volume ratio of 5:5:5:5, v/v which can get 19.7 mg Isorhamnetin of 65.64％. The second stage used HEMW, 5:5:6:4, v/v to resolve the components from the first stage. 700 mg amount of the crude extract was separated, yielding 11.8 mg at a high purity of 98％.
High-speed countercurrent chromatography Isorhamnetin stigma maydis
Yun Wei Xiaoying Cao
Applied Chemistry Department, Faculty of Sciences, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing 100029 P.R.China
国际会议
The 5th International Conference on Separation Science and Technology(第五届国际分离科学与技术会议)
北京
英文
2007-10-14（万方平台首次上网日期，不代表论文的发表时间）